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| Provedor de dados: |
International Journal of Morphology
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| País: |
Chile
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| Título: |
Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
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| Autores: |
Ili,Carmen G
Brebi,Priscilla
Garcia,Patricia
Leal,Pamela
Lopez,Jaime
Tapia,Oscar
Letelier,Pablo
Weber,Helga
Castillo,Jonathan
Roa,Juan C
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| Data: |
2015-06-01
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| Ano: |
2015
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| Palavras-chave: |
Apoptosis
Proliferation
Cervical cancer
SiRNA
C-FLIPL
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| Resumo: |
Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P<0.05), while C-33A cells did not show significant differences. Ki-67 and PCNA immunocytochemistry was performed to evaluate proliferation on these cervical cancer cell lines. SiHa cells with c-FLIPL knockdown showed elevated expression of Ki-67 protein compared with their scrambled counterparts (P<0.0001), while C-33A c-FLIPL knockdown cells showed a significantly lower in PCNA expression (P<0.01) compared with control. All three c-FLIP-transfected cell lines showed a higher level of apoptosis compared with their scrambled controls. Our results suggest that c-FLIPL could have effects in proliferation and apoptosis in cervical cancer cell lines.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036
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| Editor: |
Sociedad Chilena de Anatomía
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| Formato: |
text/html
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| Fonte: |
International Journal of Morphology v.33 n.2 2015
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